5FG8

Drosophila CaMKII-wt in complex with a fragment of the Eag potassium channel and Mg2+/ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98403
Spacegroup name	P 1 21 1
Unit cell lengths	36.503, 59.223, 70.759
Unit cell angles	90.00, 97.23, 90.00

Refinement procedure

Resolution	30.194 - 1.955
R-factor	0.1924
Rwork	0.191
R-free	0.22090
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.171
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	REFMAC
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.194	30.194	1.950
High resolution limit [Å]	1.880	7.280	1.880
Rmerge		0.033	0.681
Rmeas	0.060
Rpim	0.033	0.024	0.520
Total number of observations	77000	759	5111
Number of reflections	23764
<I/σ(I)>	12.4	28	1.5
Completeness [%]	97.2	63.7	88
Redundancy	3.2	2.7	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293.15	26% w/v PEG 4000, 0.2 M ammonium acetate, 5 mM magnesium chloride, 0.8 mM ADP, 0.1 mM sodium citrate pH 5.0.