5FG0
Structure of the conserved yeast listerin (Ltn1) N-terminal domain, MONOCLINIC FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.514, 80.352, 97.325 |
| Unit cell angles | 90.00, 116.58, 90.00 |
Refinement procedure
| Resolution | 48.347 - 2.410 |
| R-factor | 0.1774 |
| Rwork | 0.176 |
| R-free | 0.21170 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.608 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1951) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.061 | 0.416 |
| Number of reflections | 46438 | |
| <I/σ(I)> | 11.6 | 2.3 |
| Completeness [%] | 98.0 | 91 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 7% (w/v) PEG8000, 0.1 M potassium chloride, and 2.5% (v/v) glycerol |
