5FFX
S. aureus MepR G34K Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-31 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.297, 96.461, 109.486 |
| Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
| Resolution | 30.620 - 1.470 |
| R-factor | 0.1544 |
| Rwork | 0.154 |
| R-free | 0.19240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l9t |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.091 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.620 | 1.510 |
| High resolution limit [Å] | 1.470 | 1.480 |
| Rmerge | 0.084 | 0.675 |
| Number of reflections | 112058 | |
| <I/σ(I)> | 13.45 | 2.8 |
| Completeness [%] | 98.3 | 92.58 |
| Redundancy | 3.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 800mM ammonium sulfate, 100 mM citric acid/sodium hydroxide pH 4.0 |
