5FFT
Crystal Structure of Surfactant Protein-A Y221A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-07-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 97.125, 97.125, 44.576 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.308 - 2.200 |
| R-factor | 0.1728 |
| Rwork | 0.169 |
| R-free | 0.20700 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.714 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.062 | 0.036 | 0.747 |
| Total number of observations | 75389 | ||
| Number of reflections | 16339 | ||
| <I/σ(I)> | 18.7 | ||
| Completeness [%] | 99.2 | 94.8 | 99.1 |
| Redundancy | 4.6 | 4.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | Crystals were grown in hanging drops over reservoirs containing 10 mM sodium cacodylate (pH 6.0), 10 mM calcium acetate, and 10% (w/v) PEG 20,000 |
