5FFS
Crystal Structure of Surfactant Protein-A Y164A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-07-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 97.162, 97.162, 44.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.645 - 1.800 |
| R-factor | 0.1635 |
| Rwork | 0.162 |
| R-free | 0.17950 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.226 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.662 | 1.700 |
| Rmerge | 0.027 | 0.020 | 0.228 |
| Total number of observations | 120336 | ||
| Number of reflections | 26014 | ||
| <I/σ(I)> | 45.2 | ||
| Completeness [%] | 96.7 | 95.8 | 74.1 |
| Redundancy | 4.6 | 5.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | Crystals were grown in hanging drops over reservoirs containing 10 mM sodium cacodylate (pH 6.0), 10 mM calcium acetate, and 10% (w/v) PEG 20,000 |
