5FFD

CopM in the Ag-bound form (by co-crystallization)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	53.872, 85.959, 31.729
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.597 - 1.451
R-factor	0.158
Rwork	0.156
R-free	0.19260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bt5
RMSD bond length	0.008
RMSD bond angle	0.816
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.450	1.450
Rmerge		0.474
Number of reflections	26798
<I/σ(I)>	13.5	2.6
Completeness [%]	99.6	98.8
Redundancy	4.2	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1M MES 6.0, 20% PEG 2000 MME, 1mM AgNO3