5FEH

Crystal structure of PCT64_35B, a broadly neutralizing anti-HIV antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-21
Detector	PSI PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 65 2 2
Unit cell lengths	135.525, 135.525, 353.602
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.892 - 3.100
R-factor	0.2602
Rwork	0.259
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dfb
RMSD bond length	0.004
RMSD bond angle	0.765
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.892	3.170
High resolution limit [Å]	3.100	3.100
Rmerge	0.140	0.670
Number of reflections	35800
<I/σ(I)>	9.8	2.9
Completeness [%]	99.4	99.4
Redundancy	4.3	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	ammonium sulfate, ethylene glycol, PEG 3350