5FEG
Crystal structure of the dimeric allergen profilin (Hev b 8)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 101 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-08-17 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5416 |
| Spacegroup name | P 32 |
| Unit cell lengths | 58.558, 58.558, 87.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.092 - 2.802 |
| R-factor | 0.2097 |
| Rwork | 0.207 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fds |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.248 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.860 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.475 | |
| Number of reflections | 8075 | |
| <I/σ(I)> | 8.9 | 2.1 |
| Completeness [%] | 98.1 | 97.6 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M TRIS-HCl, pH 9.0; 2.0 M Ammonium Sulfate |
