5FDY

Crystal structure of the Voltage-gated Sodium Channel Beta 2 subunit extracellular domain, C72A/C75A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-08
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.542
Spacegroup name	P 1
Unit cell lengths	28.386, 36.180, 59.186
Unit cell angles	89.96, 89.99, 76.36

Refinement procedure

Resolution	29.593 - 1.850
R-factor	0.1577
Rwork	0.156
R-free	0.18530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mz2
RMSD bond length	0.004
RMSD bond angle	0.648
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.219
High resolution limit [Å]	1.850	1.850
Rmerge	0.100	0.320
Number of reflections	19296
<I/σ(I)>	14.94
Completeness [%]	99.0
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	PEG 6000