5FDV

Crystal structure of the Pyrrhocoricin antimicrobial peptide bound to the Thermus thermophilus 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	209.940, 450.100, 622.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.812 - 2.800
R-factor	0.191
Rwork	0.189
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.029
RMSD bond angle	1.942
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.172	0.025	2.210
Rmeas	0.178	0.026	2.297
Total number of observations	19712065
Number of reflections	1428776	16577	105222
<I/σ(I)>	15.99	76.44	1.29
Completeness [%]	100.0	97.9	100
Redundancy	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	Tris-HCl, PEG 20000, MPD, Arginine