5FDS
Crystal structure of the monomeric allergen profilin (Hev b 8)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 101 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-02-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5416 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.037, 59.037, 132.891 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.220 - 1.900 |
| R-factor | 0.1742 |
| Rwork | 0.173 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.059 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.220 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.447 |
| Number of reflections | 19125 | |
| <I/σ(I)> | 18.9 | 3.7 |
| Completeness [%] | 98.2 | 98.2 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 0.1M TRIS-HCL, pH 9.0, 1.6 M Ammonium Sulfate |
