5FDJ
Hen egg lysozyme at room temperature solved from datasets acquired by ultrasonic acoustic levitation method and processed by CrystFEL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2015-06-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.600, 78.600, 37.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.300 - 1.800 |
| R-factor | 0.18121 |
| Rwork | 0.179 |
| R-free | 0.21998 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xjb |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.818 |
| Data reduction software | CrystFEL |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.130 | |
| Number of reflections | 11603 | |
| <I/σ(I)> | 15.9 | 10.11 |
| Completeness [%] | 99.9 | 97.4 |
| Redundancy | 406.7 | 121.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | Na acetate, MPEG 5K, NaCl, Na acetate, ethylene glycerol |
