5FD9

X-ray Crystal Structure of ESCRT-III Snf7 core domain (conformation B)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	80
Detector technology	CCD
Collection date	2015-03-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	29.901, 46.201, 44.601
Unit cell angles	90.00, 98.50, 90.00

Refinement procedure

Resolution	24.907 - 1.600
R-factor	0.2106
Rwork	0.210
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4abm
RMSD bond length	0.006
RMSD bond angle	0.808
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.600	1.550
Rmerge		0.078
Number of reflections	16849
<I/σ(I)>	8.85	1.35
Completeness [%]	95.8	90.65
Redundancy	4.4	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	110mM NaCl, 70mM MES:NaOH pH5.5, 6% PEG20,000