5FD7
X-ray Crystal Structure of ESCRT-III Snf7 core domain (conformation A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-03-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.451, 52.196, 54.486 |
| Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
| Resolution | 27.009 - 2.400 |
| R-factor | 0.2593 |
| Rwork | 0.259 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4abm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.239 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.088 | 0.249 |
| Number of reflections | 6376 | |
| <I/σ(I)> | 8.04 | 2.91 |
| Completeness [%] | 98.0 | 93.72 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 100mM NaCl, 100mM MES:NaOH pH5.5, 3% PEG20,000 |
