5FD6

zinc-bound manganese uptake regulator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2003-09-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.933
Spacegroup name	P 43
Unit cell lengths	94.950, 94.950, 63.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.460 - 2.480
R-factor	0.18891
Rwork	0.187
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.429
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.900	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.092	0.260
Number of reflections	19841
<I/σ(I)>	9.1	4.2
Completeness [%]	99.7	99.4
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Trizma, pH 8.0, 1.5 M ammonium sulfate. The protein sample contained a 10:1 molar excess of Zn(II)