5FD5
manganese uptake regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 163.800, 171.320, 90.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.920 - 1.910 |
| R-factor | 0.22337 |
| Rwork | 0.222 |
| R-free | 0.24660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.101 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.036 | 0.540 |
| Number of reflections | 97820 | |
| <I/σ(I)> | 29.6 | 2.2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate pH 4.75, 0.8 M ammonium sulfate and 2 M NaCl |
