5FD0

Streptomyces plicatus N-acetyl-beta-hexosaminidase in complex with NAGlucal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-05-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	133.170, 133.170, 176.525
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.759 - 2.000
R-factor	0.1506
Rwork	0.150
R-free	0.17960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hp4
RMSD bond length	0.010
RMSD bond angle	1.031
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_2236))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.810	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.123	0.704
Number of reflections	61567
<I/σ(I)>	11.1	2.3
Completeness [%]	98.2	94.1
Redundancy	7.2	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	1.8M ammonium sulphate, 0.1M trisodium citrate pH 6.0