5FCP
X-RAY STRUCTURE OF THE ADDUCT BETWEEN HEN EGG WHITE LYSOZYME AND CISPLATIN AT LONG INCUBATION TIMES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0650 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.280, 79.280, 37.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.060 - 1.550 |
| R-factor | 0.17005 |
| Rwork | 0.168 |
| R-free | 0.21289 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.980 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.060 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.095 | 1.005 |
| Number of reflections | 18069 | |
| <I/σ(I)> | 11.9 | 0.8 |
| Completeness [%] | 97.6 | 99.1 |
| Redundancy | 5.7 | 5.8 |
| CC(1/2) | 0.273 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Crystals were grown using a reservoir solution of 1.1 M NaCl, 0.1 M sodium acetate pH 4.4 and a drop of 1 uL adduct solution and 1 uL reservoir solution. |
