5FCF
Crystal Structure of Xaa-Pro dipeptidase from Xanthomonas campestris, phosphate and Mn bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE BL-21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | BL-21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.962, 104.284, 112.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 1.850 |
| R-factor | 0.16088 |
| Rwork | 0.159 |
| R-free | 0.19591 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.369 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.290 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.102 | 0.871 |
| Number of reflections | 82603 | |
| <I/σ(I)> | 14 | 2.4 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 294 | 40mM KH2PO4, 20% Glycerol, 14% PEG 8000, 2mM MnCl2 |
