5FC8

Mouse importin alpha: Dengue 3 NS5 C-terminal NLS peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-28
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.940, 89.880, 100.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.350 - 2.100
R-factor	0.1838
Rwork	0.182
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ul1
RMSD bond length	0.010
RMSD bond angle	1.339
Data reduction software	MOSFLM
Data scaling software	Aimless (ccp4-6.5)
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.350
High resolution limit [Å]	2.100
Rmerge	0.087
Number of reflections	41892
<I/σ(I)>	10.5
Completeness [%]	99.1
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296	1 M sodium citrate, 10 mM DTT