5FC2
Structure of a separase in complex with a pAMK peptide containing a phospho-serine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.354, 85.013, 119.268 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.510 - 1.840 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.20700 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.047 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.838 |
| Number of reflections | 50208 |
| <I/σ(I)> | 19.8 |
| Completeness [%] | 100.0 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | pentaerythritol propoxylate (5/4 PO/OH), KCl, HEPES |
