5FBS
Crystal structure of rifampin phosphotransferase RPH-Lm from Listeria monocytogenes in complex with ADP and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.12720 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.510, 58.510, 601.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.540 - 2.590 |
| R-factor | 0.2634 |
| Rwork | 0.260 |
| R-free | 0.31770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fbt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.161 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.540 | 2.660 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.107 | 0.717 |
| Number of reflections | 34559 | |
| <I/σ(I)> | 12.3 | 2.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 5 mM ADP, 20% PEG3350, 0.2 M calcium chloride, 2% glycerol |
