5FBR
PI4KB in complex with Rab11 and the MI359 Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.266, 103.937, 187.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.650 - 3.280 |
| R-factor | 0.2271 |
| Rwork | 0.225 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d0l |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.905 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.650 | 3.400 |
| High resolution limit [Å] | 3.280 | 3.280 |
| Rmerge | 0.128 | |
| Total number of observations | 95184 | |
| Number of reflections | 15037 | |
| <I/σ(I)> | 12.75 | |
| Completeness [%] | 97.4 | 76.48 |
| Redundancy | 6.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.15M amonium sulfate, 0.1M MES pH=6, 15% PEG 4000 |
