5FB5
Crystal structure of the bacteriophage phi29 tail knob protein gp9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2014-10-03 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 183.448, 183.448, 183.448 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.029 - 3.500 |
| R-factor | 0.2022 |
| Rwork | 0.201 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fei |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.225 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.029 |
| High resolution limit [Å] | 3.500 |
| Number of reflections | 26215 |
| <I/σ(I)> | 18.36 |
| Completeness [%] | 100.0 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 297.15 | 0.1M sodium acetate, 2.0M ammonium sulfate |
