5FA8

SAM complex with aKMT from the hyperthermophilic archaeon Sulfolobus islandicu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	93
Detector technology	CCD
Collection date	2012-12-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	C 1 2 1
Unit cell lengths	48.301, 57.993, 55.725
Unit cell angles	90.00, 100.93, 90.00

Refinement procedure

Resolution	28.600 - 1.300
R-factor	0.1848
Rwork	0.183
R-free	0.21240
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.222
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.600
High resolution limit [Å]	1.300
Number of reflections	69363
<I/σ(I)>	2.1
Completeness [%]	95.5
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	10mM magnesium chloride hexahydrate, 0.1M HEPES-NaOH, pH 7.0, 15%(w/v) polyethylene glycol 3,350, 5mM nickel chloride hexahydrate