5F9P

Crystal structure study of anthrone oxidase-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	77.15
Detector technology	CCD
Collection date	2013-05-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 62
Unit cell lengths	90.273, 90.273, 94.745
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.515 - 2.078
R-factor	0.1576
Rwork	0.155
R-free	0.20650
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.032
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8.2_1309)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.525	2.152
High resolution limit [Å]	2.078	2.078
Number of reflections	26423
<I/σ(I)>	5.71
Completeness [%]	100.0	99.9
Redundancy	22.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289.15	0.1M HEPES, 1.4M sodium citrate tribasic dehydrate