5F9M
Crystal structure of native B3275, member of MccF family of enzymes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.779, 70.858, 85.490 |
| Unit cell angles | 92.12, 90.24, 89.87 |
Refinement procedure
| Resolution | 37.190 - 2.402 |
| R-factor | 0.1889 |
| Rwork | 0.187 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f1y |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.598 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_1888) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.190 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.100 |
| Number of reflections | 48084 |
| <I/σ(I)> | 8 |
| Completeness [%] | 96.5 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M sodium Chloride, 0.1 M BIS-TRIS, 25% Peg 3350 |
