5F8V
Crystal structure of PLP bound phosphoserine aminotransferase (PSAT) from Trichomonas vaginalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-02-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.967, 235.434, 134.520 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 37.420 - 2.140 |
| R-factor | 0.21022 |
| Rwork | 0.209 |
| R-free | 0.23905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.253 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.440 | |
| Number of reflections | 177429 | |
| <I/σ(I)> | 7.9 | 3.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 25-30% PEG 3350 (w/v),100mM sodium acetate pH 4.5-5.0, 200mM sodium malonate, 10% glycerol (v/v) |
