5F8N

Enterovirus 71 Polymerase Elongation Complex (C3S6 Form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97892
Spacegroup name	P 21 21 21
Unit cell lengths	63.723, 77.583, 151.435
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.524 - 2.484
R-factor	0.196
Rwork	0.194
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ol6
RMSD bond length	0.008
RMSD bond angle	1.126
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (phenix.refine: 1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	2.560
High resolution limit [Å]	2.470	5.320	2.470
Rmerge	0.047	0.022	0.506
Rmeas	0.053	0.025	0.585
Rpim	0.025	0.011	0.284
Total number of observations	104950
Number of reflections	25817
<I/σ(I)>	13.3
Completeness [%]	95.1	92.3	94.8
Redundancy	4.1	4.5	3.9
CC(1/2)		0.999	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.085M MES pH 6.5, 25.5%(w/v) PEG 5000 monomethyl ether, 15%(v/v) Glycerol