5F8N
Enterovirus 71 Polymerase Elongation Complex (C3S6 Form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.723, 77.583, 151.435 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.524 - 2.484 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ol6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.126 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (phenix.refine: 1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.000 | 60.000 | 2.560 |
| High resolution limit [Å] | 2.470 | 5.320 | 2.470 |
| Rmerge | 0.047 | 0.022 | 0.506 |
| Rmeas | 0.053 | 0.025 | 0.585 |
| Rpim | 0.025 | 0.011 | 0.284 |
| Total number of observations | 104950 | ||
| Number of reflections | 25817 | ||
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 95.1 | 92.3 | 94.8 |
| Redundancy | 4.1 | 4.5 | 3.9 |
| CC(1/2) | 0.999 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.085M MES pH 6.5, 25.5%(w/v) PEG 5000 monomethyl ether, 15%(v/v) Glycerol |
