5F8K

Crystal structure of the Bac7(1-16) antimicrobial peptide bound to the Thermus thermophilus 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	90
Detector technology	CCD
Collection date	2015-07-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	209.960, 450.130, 622.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.625 - 2.800
R-factor	0.2509
Rwork	0.249
R-free	0.29160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.019
RMSD bond angle	1.073
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			2.970
High resolution limit [Å]	2.800	8.290	2.800
Rmerge	0.512	0.076	2.339
Rmeas	0.545	0.082	2.496
Total number of observations	11868574
Number of reflections	1422951	56511	223820
<I/σ(I)>	5.71	22.6	0.96
Completeness [%]	99.6	99.3	97.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	Tris-HCl, PEG 20000, MPD, Arginine