5F8G
Enterovirus 71 Polymerase Elongation Complex (C1S1 Form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.128, 77.630, 153.649 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.774 - 2.780 |
| R-factor | 0.1952 |
| Rwork | 0.193 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ol6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.125 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.000 | 60.000 | 2.880 |
| High resolution limit [Å] | 2.780 | 5.990 | 2.780 |
| Rmerge | 0.117 | 0.051 | 0.499 |
| Rmeas | 0.129 | 0.056 | 0.551 |
| Rpim | 0.054 | 0.024 | 0.229 |
| Total number of observations | 104922 | ||
| Number of reflections | 19258 | ||
| <I/σ(I)> | 6.8 | ||
| Completeness [%] | 97.6 | 94.9 | 98.4 |
| Redundancy | 5.4 | 5.3 | 5.5 |
| CC(1/2) | 0.998 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.085 M MES pH 6.5, 25.5 % (w/v) PEG 5000 monomethyl ether, 15 %(v/v) Glycerol |
