5F82
Apo GES-5 C69G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-27 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9495 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.711, 80.939, 71.052 |
| Unit cell angles | 90.00, 101.36, 90.00 |
Refinement procedure
| Resolution | 37.192 - 0.960 |
| R-factor | 0.1187 |
| Rwork | 0.118 |
| R-free | 0.13390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.399 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 0.980 |
| High resolution limit [Å] | 0.960 | 0.960 |
| Rmerge | 0.502 | |
| Number of reflections | 280826 | |
| <I/σ(I)> | 13.6 | 2.2 |
| Completeness [%] | 97.6 | 73.5 |
| Redundancy | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 278 | 0.2 M D,L-malic acid, 20% PEG3350 |
