5F74
Crystal structure of ChREBP:14-3-3 complex bound with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2015-07-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 115.620, 115.620, 59.372 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.419 - 2.350 |
| R-factor | 0.1914 |
| Rwork | 0.188 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gnt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.995 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.350 |
| Rmerge | 0.041 |
| Number of reflections | 17340 |
| <I/σ(I)> | 22.9 |
| Completeness [%] | 100.0 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM sodium citrate 200 mM sodium potassium tartrate 2.2 M ammonium sulfate |
