5F6Z

Sandercyanin Fluorescent Protein purified from Sander vitreus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-08-15
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	93.394, 93.394, 246.426
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.835 - 2.248
R-factor	0.2398
Rwork	0.237
R-free	0.31160
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.288
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	d*TREK
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.840	2.329
High resolution limit [Å]	2.248	2.248
Number of reflections	52052
<I/σ(I)>	5.86
Completeness [%]	98.6
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	277	0.1M sodium acetate, 3M magnesium sulphate hydrate, 20% PEG 8000
1	VAPOR DIFFUSION, HANGING DROP	7.4	277	0.1M sodium acetate, 3M magnesium sulphate hydrate, 20% PEG 8000