5F6I
Crystal Structure of Tier 2 Neutralizing Antibody DH428 from a Rhesus Macaque
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 73.106, 74.628, 102.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.356 - 2.320 |
| R-factor | 0.2204 |
| Rwork | 0.218 |
| R-free | 0.26460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | separated Fv and Fc regions of I3.2 Fab from PDB Entry 4QHL |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.278 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.360 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.086 | 0.744 |
| Number of reflections | 22905 | |
| <I/σ(I)> | 13.43 | 2.2 |
| Completeness [%] | 91.7 | 95 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 40% PEG 400 and 100 mM sodium citrate, pH 5.0 |
