5F69

Drosophila Melanogaster Cycle W398A PAS-B with Bound Glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-15
Detector	ADSC QUANTUM 210
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	73.298, 44.398, 38.502
Unit cell angles	90.00, 114.75, 90.00

Refinement procedure

Resolution	22.199 - 1.374
R-factor	0.1722
Rwork	0.170
R-free	0.19760
RMSD bond length	0.013
RMSD bond angle	1.263
Data scaling software	HKL-2000
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.390
High resolution limit [Å]	1.370	3.720	1.370
Rmerge	0.089	0.075	0.538
Rmeas	0.106	0.089	0.666
Rpim	0.057	0.048	0.387
Total number of observations	75736
Number of reflections	23120
<I/σ(I)>	5.9
Completeness [%]	98.3	100	77.6
Redundancy	3.3	3.3	2.4
CC(1/2)		0.986	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Ammonium Sulfate