5F68

Drosophila Melanogaster Cycle W398A PAS-B with Bound Ethylene Glycol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-14
Detector	ADSC QUANTUM 210
Wavelength(s)	.987
Spacegroup name	C 1 2 1
Unit cell lengths	73.308, 44.169, 38.574
Unit cell angles	90.00, 115.16, 90.00

Refinement procedure

Resolution	34.915 - 1.230
R-factor	0.181
Rwork	0.180
R-free	0.19610
RMSD bond length	0.012
RMSD bond angle	1.266
Data scaling software	HKL-2000
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.250
High resolution limit [Å]	1.230	3.340	1.230
Rmerge	0.102	0.084	0.541
Rmeas	0.121	0.100	0.695
Rpim	0.066	0.053	0.430
Total number of observations	95323
Number of reflections	30239
<I/σ(I)>	5
Completeness [%]	93.3	100	63.9
Redundancy	3.2	3.4	2
CC(1/2)		0.969	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Ammonium Sulfate