5F67
An exquisitely specific PDZ/target recognition revealed by the structure of INAD PDZ3 in complex with TRP channel tail
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-10 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 57.420, 30.126, 61.454 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 41.770 - 1.760 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3diw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.116 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.056 | 0.190 |
| Number of reflections | 21025 | |
| <I/σ(I)> | 34.9 | 9.5 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2%(v/v) Polyethylene glycol 400, 0.1 M Imidazole pH 7.0, 24%(w/v) Polyethylene glycol monomethyl ether 5000 |
