5F5V

Crystal structure of the Snu23-Prp38-MFAP1(217-296) complex of Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 32
Unit cell lengths	84.810, 84.810, 96.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.405 - 3.100
R-factor	0.2979
Rwork	0.295
R-free	0.34730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rz9
RMSD bond length	0.003
RMSD bond angle	0.845
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.410	3.180
High resolution limit [Å]	3.100	3.100
Number of reflections	14056
<I/σ(I)>	21.2	1.2
Completeness [%]	99.8	99.2
Redundancy	10.2	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	10	277.15	0.1 M CAPS, pH 10.0, and 30 % (w/v) PEG 300