5F5M

Crystal structure of Marburg virus nucleoprotein core domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 3 2 1
Unit cell lengths	147.959, 147.959, 58.982
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.990 - 2.902
R-factor	0.2149
Rwork	0.212
R-free	0.26910
RMSD bond length	0.003
RMSD bond angle	0.596
Data reduction software	SCALEPACK
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.900
Number of reflections	16663
<I/σ(I)>	27.75
Completeness [%]	99.9
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	100mM HEPES(pH7.5), 200mM calcium chloride, 14% (v/v) PEG 400