5F55

Structure of RecJ complexed with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2015-05-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	105.820, 105.820, 165.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.330 - 2.600
R-factor	0.1873
Rwork	0.185
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zxp
RMSD bond length	0.005
RMSD bond angle	0.849
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: 000))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000		2.660
High resolution limit [Å]	2.600	11.620	2.600
Rmerge	0.065	0.030	0.791
Rmeas	0.069	0.033	0.844
Total number of observations	280581
Number of reflections	33594	364	2454
<I/σ(I)>	22.61	46.14	3.04
Completeness [%]	99.6	82.5	99.4
Redundancy	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	MES, MnCl2, Li2SO4