5F4V

Crystal structure of the human sperm Izumo1 residues 22-268

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-30
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	H 3
Unit cell lengths	121.300, 121.300, 53.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.603 - 2.900
R-factor	0.1907
Rwork	0.188
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f4e
RMSD bond length	0.012
RMSD bond angle	1.270
Data reduction software	XDS
Data scaling software	Aimless (0.5.8)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.603	47.600	3.080
High resolution limit [Å]	2.900	8.700	2.900
Rmerge	0.120	0.041	0.526
Rpim	0.055	0.019	0.239
Total number of observations	37350	1351	5979
Number of reflections	6484
<I/σ(I)>	12.9	33.2	3.7
Completeness [%]	100.0	99.4	100
Redundancy	5.8	5.7	5.8
CC(1/2)	0.996	0.998	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.085 M HEPES sodium salt (pH 7.5), 8.5% (v/v) isopropanol, 17% (w/v) PEG 4000 and 15% (v/v) glycerol