5F48
Crystal structure of an aminoglycoside acetyltransferase meta-AAC0020 from an uncultured soil metagenomic sample in complex with coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.133, 80.187, 53.926 |
| Unit cell angles | 90.00, 91.97, 90.00 |
Refinement procedure
| Resolution | 23.621 - 1.950 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | apoenzyme |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.243 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.085 | 0.611 |
| Number of reflections | 27961 | |
| <I/σ(I)> | 25.85 | |
| Completeness [%] | 97.6 | 96.2 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 9% PEG 8000, 0.2 M calcium acetate hydrate, 0.1 M sodium cacodylate, 20 mM gentamicin, 20 acetyl-CoA |
