5F3Y

Crystal Structure of Myo7b N-MyTH4-FERM-SH3 in complex with Anks4b CEN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9788
Spacegroup name	P 41 21 2
Unit cell lengths	197.516, 197.516, 97.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.718 - 3.409
R-factor	0.2432
Rwork	0.241
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pvl
RMSD bond length	0.008
RMSD bond angle	1.111
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.460
High resolution limit [Å]	3.400	9.210	3.400
Rmerge	0.100	0.031	0.888
Rmeas	0.109	0.033	0.987
Rpim	0.040	0.011	0.419
Total number of observations	169291
Number of reflections	24972
<I/σ(I)>	8.7
Completeness [%]	92.9	84.2	95.7
Redundancy	6.8	8.2	4.9
CC(1/2)		0.999	0.657

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	25%(w/v) Pentaerythritol ethoxylate (3/4 EO/OH), 0.1 M MES