5F3X
Crystal structure of Harmonin NPDZ1 in complex with ANKS4B SAM-PBM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 32 |
| Unit cell lengths | 124.566, 124.566, 49.654 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.511 - 2.649 |
| R-factor | 0.2291 |
| Rwork | 0.227 |
| R-free | 0.26440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k1r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.994 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.511 | 2.700 |
| High resolution limit [Å] | 2.649 | 2.650 |
| Number of reflections | 24978 | |
| <I/σ(I)> | 38.2 | 2.9 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 5.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 3.0 M NaCl, 0.1 M Bis-tris |
