5F3Q

Crystal structure of a noncanonical Dicer protein from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	43.281, 91.015, 102.746
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.996 - 2.100
R-factor	0.2039
Rwork	0.202
R-free	0.24640
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.925
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	24145
<I/σ(I)>	24.2
Completeness [%]	99.1
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	1.26 M (NH4)2SO4, 0.1 M Hepes pH 7.8