5F2Z
Crystal structure of membrane associated PatA from Mycobacterium smegmatis in complex with palmitate - P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.830, 84.480, 98.800 |
| Unit cell angles | 90.00, 110.78, 90.00 |
Refinement procedure
| Resolution | 92.370 - 2.900 |
| R-factor | 0.2407 |
| Rwork | 0.239 |
| R-free | 0.27370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f2t |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.995 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.370 | |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.115 | |
| Number of reflections | 23420 | |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 96.2 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100 mM Tris-HCl pH 7.0, 230 mM MgCl2 and 12-16% (w/v) PEG 8,000 |
