5F2T
Crystal structure of membrane associated PatA from Mycobacterium smegmatis in complex with palmitate - C 2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.600, 71.090, 76.250 |
| Unit cell angles | 90.00, 91.95, 90.00 |
Refinement procedure
| Resolution | 56.601 - 2.060 |
| R-factor | 0.1761 |
| Rwork | 0.173 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.768 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2219: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.600 | 2.110 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.068 | 0.410 |
| Number of reflections | 30533 | |
| <I/σ(I)> | 10.6 | 2 |
| Completeness [%] | 98.5 | 86.7 |
| Redundancy | 3.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 100 mM Tris-HCl pH 7.0, 230 mM MgCl2 and 12-16% (w/v) PEG 8,000 |
