5F29

Structure of RCK domain with cda

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-13
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	41.489, 47.297, 70.174
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.744 - 1.821
R-factor	0.2174
Rwork	0.201
R-free	0.25530
RMSD bond length	0.008
RMSD bond angle	1.202
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	23.500
High resolution limit [Å]	1.810
Number of reflections	12747
<I/σ(I)>	22
Completeness [%]	98.9
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	Tris 0.1M pH 7.0 PEG 4000 15%