5F18
Structural basis of Ebola virus entry: viral glycoprotein bound to its endosomal receptor Niemann-Pick C1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.038 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 60.006, 60.006, 270.347 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.043 - 2.000 |
| R-factor | 0.1839 |
| Rwork | 0.182 |
| R-free | 0.21900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 20721 |
| <I/σ(I)> | 29 |
| Completeness [%] | 99.9 |
| Redundancy | 20.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.07M sodium acetate trihydrate, pH 4.6, 5.6% (w/v) polyethylene glycol 4000, 30% (v/v) glycerol |
